- Human Inter-alpha-trypsin inhibitor heavy chain H4(ITIH4/IHRP/ITIHL1/PK120/PRO1851)ELISA Kit
- CUSABIO Technology LLC
- Pricing InfoSupplier PageView Company Product Page
- CSB-E17022h
- ELISA
- ELISA Kit
- Homo sapiens (Human)
- inter-alpha-trypsin inhibitor heavy chain 4
- GP120, H4P, IHRP, ITI-HC4, ITIHL1, PK-120, PK120
- Immunology
- 1-5h
- inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
- 50-100ul
- 0.156 ng/mL
- serum, plasma, tissue homogenates
- Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
- 450 nm
- 0.625 ng/mL-40 ng/mL
- 96T
Specifications/Features
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITIH4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITIH4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITIH4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITIH4 bound in the initial step. The color development is stopped and the intensity of the color is measured.